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infection. Cell lysates of persistently Mtb -infected THP-1 macrophages (Figure ) and the corresponding non-infected controls were used for <t>kinome</t> analysis <t>using</t> <t>Kinexus</t> antibody arrays. A. The 74 differentially expressed signaling proteins were plotted ranked according to their Z-ratio, which indicates statistically significant up- (red) or down-regulation effects (green). The most prominent signal was that of Protein Phosphatase, Mg 2+ /Mn 2+ -dependent 1A (PPM1A) expression (Z-ratio = 8.6). B. Pie-chart showing the relative numbers of identified altered signaling proteins and their association with different functional categories or pathways. C. PPM1A protein expression levels from lysates of Mtb -infected THP-1 cells or D. primary human monocytes at various days post Mtb -infection were analyzed by Western blotting. Densitometry analysis was performed by ImageJ to quantify PPM1A band intensities as normalized to α-tubulin or GAPDH, and fold changes relative to day 0 post infection are reported.
Antibody Based Kinome Arrays, supplied by Kinexus Bioinformatics Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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infection. Cell lysates of persistently Mtb -infected THP-1 macrophages (Figure ) and the corresponding non-infected controls were used for <t>kinome</t> analysis <t>using</t> <t>Kinexus</t> antibody arrays. A. The 74 differentially expressed signaling proteins were plotted ranked according to their Z-ratio, which indicates statistically significant up- (red) or down-regulation effects (green). The most prominent signal was that of Protein Phosphatase, Mg 2+ /Mn 2+ -dependent 1A (PPM1A) expression (Z-ratio = 8.6). B. Pie-chart showing the relative numbers of identified altered signaling proteins and their association with different functional categories or pathways. C. PPM1A protein expression levels from lysates of Mtb -infected THP-1 cells or D. primary human monocytes at various days post Mtb -infection were analyzed by Western blotting. Densitometry analysis was performed by ImageJ to quantify PPM1A band intensities as normalized to α-tubulin or GAPDH, and fold changes relative to day 0 post infection are reported.
Antibody Array Kamtm, supplied by Kinexus Bioinformatics Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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infection. Cell lysates of persistently Mtb -infected THP-1 macrophages (Figure ) and the corresponding non-infected controls were used for <t>kinome</t> analysis <t>using</t> <t>Kinexus</t> antibody arrays. A. The 74 differentially expressed signaling proteins were plotted ranked according to their Z-ratio, which indicates statistically significant up- (red) or down-regulation effects (green). The most prominent signal was that of Protein Phosphatase, Mg 2+ /Mn 2+ -dependent 1A (PPM1A) expression (Z-ratio = 8.6). B. Pie-chart showing the relative numbers of identified altered signaling proteins and their association with different functional categories or pathways. C. PPM1A protein expression levels from lysates of Mtb -infected THP-1 cells or D. primary human monocytes at various days post Mtb -infection were analyzed by Western blotting. Densitometry analysis was performed by ImageJ to quantify PPM1A band intensities as normalized to α-tubulin or GAPDH, and fold changes relative to day 0 post infection are reported.
Antibody Based Protein Arrays Kinextm, supplied by Kinexus Bioinformatics Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody based protein arrays kinextm/product/Kinexus Bioinformatics Corporation
Average 90 stars, based on 1 article reviews
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Kinexus Bioinformatics Corporation kinase antibody arrays
infection. Cell lysates of persistently Mtb -infected THP-1 macrophages (Figure ) and the corresponding non-infected controls were used for <t>kinome</t> analysis <t>using</t> <t>Kinexus</t> antibody arrays. A. The 74 differentially expressed signaling proteins were plotted ranked according to their Z-ratio, which indicates statistically significant up- (red) or down-regulation effects (green). The most prominent signal was that of Protein Phosphatase, Mg 2+ /Mn 2+ -dependent 1A (PPM1A) expression (Z-ratio = 8.6). B. Pie-chart showing the relative numbers of identified altered signaling proteins and their association with different functional categories or pathways. C. PPM1A protein expression levels from lysates of Mtb -infected THP-1 cells or D. primary human monocytes at various days post Mtb -infection were analyzed by Western blotting. Densitometry analysis was performed by ImageJ to quantify PPM1A band intensities as normalized to α-tubulin or GAPDH, and fold changes relative to day 0 post infection are reported.
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Kinexus Bioinformatics Corporation array 359 phosphosite-specific antibody array
infection. Cell lysates of persistently Mtb -infected THP-1 macrophages (Figure ) and the corresponding non-infected controls were used for <t>kinome</t> analysis <t>using</t> <t>Kinexus</t> antibody arrays. A. The 74 differentially expressed signaling proteins were plotted ranked according to their Z-ratio, which indicates statistically significant up- (red) or down-regulation effects (green). The most prominent signal was that of Protein Phosphatase, Mg 2+ /Mn 2+ -dependent 1A (PPM1A) expression (Z-ratio = 8.6). B. Pie-chart showing the relative numbers of identified altered signaling proteins and their association with different functional categories or pathways. C. PPM1A protein expression levels from lysates of Mtb -infected THP-1 cells or D. primary human monocytes at various days post Mtb -infection were analyzed by Western blotting. Densitometry analysis was performed by ImageJ to quantify PPM1A band intensities as normalized to α-tubulin or GAPDH, and fold changes relative to day 0 post infection are reported.
Array 359 Phosphosite Specific Antibody Array, supplied by Kinexus Bioinformatics Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/array 359 phosphosite-specific antibody array/product/Kinexus Bioinformatics Corporation
Average 90 stars, based on 1 article reviews
array 359 phosphosite-specific antibody array - by Bioz Stars, 2026-03
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infection. Cell lysates of persistently Mtb -infected THP-1 macrophages (Figure ) and the corresponding non-infected controls were used for kinome analysis using Kinexus antibody arrays. A. The 74 differentially expressed signaling proteins were plotted ranked according to their Z-ratio, which indicates statistically significant up- (red) or down-regulation effects (green). The most prominent signal was that of Protein Phosphatase, Mg 2+ /Mn 2+ -dependent 1A (PPM1A) expression (Z-ratio = 8.6). B. Pie-chart showing the relative numbers of identified altered signaling proteins and their association with different functional categories or pathways. C. PPM1A protein expression levels from lysates of Mtb -infected THP-1 cells or D. primary human monocytes at various days post Mtb -infection were analyzed by Western blotting. Densitometry analysis was performed by ImageJ to quantify PPM1A band intensities as normalized to α-tubulin or GAPDH, and fold changes relative to day 0 post infection are reported.

Journal: Oncotarget

Article Title: Protein Phosphatase, Mg 2+ /Mn 2+ -dependent 1A controls the innate antiviral and antibacterial response of macrophages during HIV-1 and Mycobacterium tuberculosis infection

doi: 10.18632/oncotarget.8190

Figure Lengend Snippet: infection. Cell lysates of persistently Mtb -infected THP-1 macrophages (Figure ) and the corresponding non-infected controls were used for kinome analysis using Kinexus antibody arrays. A. The 74 differentially expressed signaling proteins were plotted ranked according to their Z-ratio, which indicates statistically significant up- (red) or down-regulation effects (green). The most prominent signal was that of Protein Phosphatase, Mg 2+ /Mn 2+ -dependent 1A (PPM1A) expression (Z-ratio = 8.6). B. Pie-chart showing the relative numbers of identified altered signaling proteins and their association with different functional categories or pathways. C. PPM1A protein expression levels from lysates of Mtb -infected THP-1 cells or D. primary human monocytes at various days post Mtb -infection were analyzed by Western blotting. Densitometry analysis was performed by ImageJ to quantify PPM1A band intensities as normalized to α-tubulin or GAPDH, and fold changes relative to day 0 post infection are reported.

Article Snippet: The utilized Kinexus antibody-based kinome arrays provide information on changes in the expression or phosphorylation state of 309 protein kinases, 38 protein phosphatases, 37 stress response proteins, 24 transcription factors, and 109 proteins involved in other signaling pathways.

Techniques: Infection, Expressing, Functional Assay, Western Blot